Evidence of Anti-Proliferative Activities in Blue Mussel (Mytilus edulis) By-Products
Open Access
- 26 March 2013
- journal article
- research article
- Published by MDPI AG in Marine Drugs
- Vol. 11 (4), 975-990
- https://doi.org/10.3390/md11040975
Abstract
Shellfish waste components contain significant levels of high quality protein and are therefore a potential source for biofunctional high-value peptides. The feasibility of applying a pilot scale enzymatic hydrolysis process to whole Mytilus edulis and, by fractionation, recover hydrolysates presenting a biological activity of interest, was evaluated. Fractions were tested on four immortalized cancerous cell lines: A549, BT549, HCT15 and PC3. The 50 kDa fraction, enriched in peptides, presented anti-proliferative activity with all cell lines and results suggest a bioactive molecule synergy within the fraction. At a protein concentration of 44 µg/mL, the 50 kDa fraction induced a mortality of 90% for PC3, 89% for A549, 85% for HCT15 and of 81% for BT549 cell lines. At the low protein concentration of only 11 µg/mL the 50 kDa fraction still entails a cell mortality of 76% for A549 and 87% for PC3 cell lines. The 50 kDa fraction contains 56% of proteins, 3% of lipids and 6% of minerals on a dry weight basis and the lowest levels detected of taurine and methionine and highest levels of threonine, proline and glycine amino acids. The enzymatic hydrolysis process suggests that Mytilus edulis by-products should be viewed as high-valued products with strong potential as anti-proliferative agent and promising active ingredients in functional foods.Keywords
This publication has 49 references indexed in Scilit:
- Bioactivity of theMurexHomeopathic Remedy and of Extracts from an Australian Muricid Mollusc against Human Cancer CellsEvidence-Based Complementary and Alternative Medicine, 2011
- Marine pharmacology in 2005–2006: Antitumour and cytotoxic compoundsEuropean Journal Of Cancer, 2008
- Nutritional composition of soluble and insoluble fractions obtained by enzymatic hydrolysis of fish-raw materialsProcess Biochemistry, 2008
- Understanding Marine Mussel AdhesionMarine Biotechnology, 2007
- Characterization of reproduction-specific genes in a marine bivalve mollusc: Influence of maturation stage and sex on mRNA expressionGene, 2007
- Natural Products as Sources of New Drugs over the Period 1981−2002Journal of Natural Products, 2003
- Studies on the nitrogen recovery in enzymic hydrolysis of Atlantic salmon (Salmo salar, L.) frames by Protamex™ proteaseProcess Biochemistry, 2002
- New Colorimetric Cytotoxicity Assay for Anticancer-Drug ScreeningJNCI Journal of the National Cancer Institute, 1990
- METABOLISM OF SULFUR AMINO ACIDS IN MYTILUS EDULIS AND RANGIA CUNEATAThe Biological Bulletin, 1960
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959