Norepinephrine Facilitates the Development of the Murine Sweat Response But Is Not Essential
Open Access
- 1 June 1997
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 17 (11), 4275-4281
- https://doi.org/10.1523/jneurosci.17-11-04275.1997
Abstract
During development, the sympathetic neurons innervating sweat glands undergo a neurotransmitter switch from noradrenergic to cholinergic between postnatal day (P) 4, when the sympathetic neurons first contact the sweat glands, and P21. Several in vitro experiments suggest that norepinephrine (NE), produced by sympathetic neurons, stimulates sweat glands to produce a factor that then induces the phenotypic switch. We tested this hypothesis in vivo using dopamine β-hydroxylase-deficient mice (DBH −/−), which are unable to synthesize NE and epinephrine, and tyrosine hydroxylase-deficient mice (TH −/−), which are unable to synthesize any catecholamines. The cholinergic agonist pilocarpine and electrostimulation of the sciatic nerve both elicited a sweat response in adult DBH −/− mice that was indistinguishable from the response of controls, and the cholinergic antagonist atropine effectively blocked these responses. We did note, however, a 1- to 2-week delay in the acquisition of the sweat response in DBH −/− mice. Although diminished in magnitude, a sweat response to pilocarpine was also noted in TH −/− mice at P21. Immunohistochemistry demonstrated that TH and vasoactive intestinal peptide were detectable at P14 and increased to adult levels by P21 in DBH +/− and DBH −/− mice. These observations indicate that NE is not essential for the acquisition of the cholinergic phenotype, but it may facilitate its postnatal development.Keywords
This publication has 23 references indexed in Scilit:
- P2x receptors bring new structure to ligand-gated ion channelsTrends in Neurosciences, 1995
- Noradrenaline is essential for mouse fetal developmentNature, 1995
- Targeted disruption of the tyrosine hydroxylase gene reveals that catecholamines are required for mouse fetal developmentNature, 1995
- The Role of Acetylcholine in Regulating Secretory Responsiveness in Rat Sweat GlandsMolecular and Cellular Neuroscience, 1995
- Innervation of footpads of normal and mutant mice lacking sweat glandsJournal of Comparative Neurology, 1994
- Acquisition of cholinergic and peptidergic properties by sympathetic innervation of rat sweat glands requires interaction with normal targetNeuron, 1990
- Evidence for neurotransmitter plasticity in vivoDevelopmental Biology, 1988
- Development and properties of the secretory response in rat sweat glands: Relationship to the induction of cholinergic function in sweat gland innervationDevelopmental Biology, 1987
- Evidence for neurotransmitter plasticity in vivo: Developmental changes in properties of cholinergic sympathetic neuronsDevelopmental Biology, 1983
- A Simple and Accurate Method for Detecting the Secretion of SweatThe Tohoku Journal of Experimental Medicine, 1948