Antipeptide antibodies directed against the C‐terminus of protein kinase Cζ (PKCζ) react with a Ca2+‐ and TPA‐sensitive PKC in HT‐29 human intestinal epithelial cells
- 16 May 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 344 (2-3), 161-165
- https://doi.org/10.1016/0014-5793(94)00379-3
Abstract
We have studied the PKC isoforms present in HT-29 M6 colon cancer cells, the differentiation of which to mucus-secreting cells is blocked by TPA. In addition to a major 72 kDa band, a 77 kDa PKC isoform was recognized by two different antibodies raised against a C-terminus-specific peptide for the TPA-insensitive isoform, PKCζ. By different criteria (association to the membrane, down-regulation, PKC activity in immunoprecipitates) we conclude that, contrary to the 72 kDa band, the 77 kDa band corresponds to a Ca2+- and TPA-sensitive PKC. These results suggest that antipeptide antibodies directed against the C-terminus of PKCζ react in human cells with a member of the conventional PKC subfamily besides PKCζ. Therefore, the data indicating that PKCζ is sensitive to different agents in various cell lines should be carefully re-evaluated.Keywords
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