Enhanced toxicity of ‘bulk' titanium dioxide compared to ‘fresh' and ‘aged' nano-TiO2in marine mussels (Mytilus galloprovincialis)
- 15 April 2013
- journal article
- research article
- Published by Informa UK Limited in Nanotoxicology
- Vol. 8 (5), 549-558
- https://doi.org/10.3109/17435390.2013.807446
Abstract
Marine bivalves (Mytilus galloprovincialis) were exposed to titanium dioxide (10 mg L−1) either as engineered nanoparticles (nTiO2; fresh, or aged under simulated sunlight for 7 days) or the bulk equivalent. Inductively coupled plasma-optical emission spectrometry analyses of mussel tissues showed higher Ti accumulation (>10-fold) in the digestive gland compared to gills. Nano-sized TiO2 showed greater accumulation than bulk, irrespective of ageing, particularly in digestive gland (>sixfold higher). Despite this, transcriptional expression of metallothionein genes, histology and histochemical analysis suggested that the bulk material was more toxic. Haemocytes showed significantly enhanced DNA damage, determined by the modified comet assay, for all treatments compared to the control, but no significant differences between the treatments. Our integrated study suggests that for this ecologically relevant organism photocatalytic ageing of nTiO2 does not significantly alter toxicity, and that bulk TiO2 may be less ecotoxicologically inert than previously assumed.Keywords
This publication has 65 references indexed in Scilit:
- Determination of hypoxia and dietary copper mediated sub-lethal toxicity in carp, Cyprinus carpio, at different levels of biological organisationChemosphere, 2012
- Practical considerations for conducting ecotoxicity test methods with manufactured nanomaterials: what have we learnt so far?Ecotoxicology, 2012
- Titanium Dioxide Nanoparticles in Food and Personal Care ProductsEnvironmental Science & Technology, 2012
- A multiple biomarker approach to investigate the effects of copper on the marine bivalve mollusc, Mytilus edulisEcotoxicology and Environmental Safety, 2011
- Ecotoxicological effects of an aged TiO2 nanocomposite measured as apoptosis in the anecic earthworm Lumbricus terrestris after exposure through water, food and soilEnvironment International, 2011
- Dietary exposure to titanium dioxide nanoparticles in rainbow trout, (Oncorhynchus mykiss): no effect on growth, but subtle biochemical disturbances in the brainEcotoxicology, 2009
- Contamination of bivalve haemolymph samples by adductor muscle components: implications for biomarker studiesEcotoxicology, 2008
- Ecotoxicity of engineered nanoparticles to aquatic invertebrates: a brief review and recommendations for future toxicity testingEcotoxicology, 2008
- The effect of organic materials on the mobility and toxicity of metals in contaminated soilsApplied Geochemistry, 2007
- The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cellsMutation Research/DNA Repair, 1995