Objective: We have recently shown that pretreatment of A10 vascular smooth muscle cells (VSMC) with angiotensin II (Ang II) for 24 h enhanced the expression of Giα-proteins, however, Giα-mediated adenylyl cyclase signaling was attenuated. Since regulators of G-protein signaling (RGS) have been shown to negatively regulate the Gα-protein, we investigated the role of RGS2, in Ang II-induced attenuation of Giα-mediated signaling in A10 vascular smooth muscle cells (VSMC). Methods: A10 VSMC were incubated with Ang II (10−7 M) for different periods of time at 37 °C. The levels of G-proteins and RGS2 protein were determined by immunoblotting using specific antibodies. Adenylyl cyclase activity stimulated or inhibited by agonists was determined to examine the functions of G-proteins. Results: Ang II treatment of A10 VSMC enhanced the expression of Giα and RGS2 proteins in a time-dependent manner, the maximal expression of Giα-proteins was observed at 1 h that remained elevated up to 24 h, whereas enhanced expression of RGS2 in these cells was detected at 1 h, peaked at 2 h and returned to base line level by 8 h. The increased expression of RGS2 by Ang II was inhibited by actinomycin D. The increased expression of Giα at 30 min when the levels of RGS2 were not augmented was reflected in increased Gi functions as was demonstrated by increased inhibition of adenylyl cyclase by inhibitory hormones (receptor-dependent functions) and increased inhibition of forskolin (FSK)-stimulated adenylyl cyclase by GTPγS (receptor-independent functions). However, with increased expression of RGS2, the Gi-mediated functions started declining and were completely abolished at 2 h of treatment when the levels of RGS2 were maximally augmented. Furthermore, prior treatment of cells with RGS2 antibody, that completely attenuated Ang II-induced expression of RGS2, prevented the GTPγS-induced attenuation of FSK-stimulated adenylyl cyclase activity. In addition, treatment of the cells with Ang II for 30 min that increased the levels of Giα-protein and not of RGS2 protein resulted in the attenuation of Gsα-mediated isoproterenol-induced stimulation of adenylyl cyclase and this attenuation was restored to control level at 1 h and remained unchanged thereafter. Conclusion: These results suggest that RGS2 may be involved in short-term regulation of Ang II-induced Gi-mediated adenylyl cyclase signalling.