Discovering structural motifs using a structural alphabet: Application to magnesium-binding sites
Open Access
- 28 March 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Bioinformatics
- Vol. 8 (1), 106
- https://doi.org/10.1186/1471-2105-8-106
Abstract
For many metalloproteins, sequence motifs characteristic of metal-binding sites have not been found or are so short that they would not be expected to be metal-specific. Striking examples of such metalloproteins are those containing Mg2+, one of the most versatile metal cofactors in cellular biochemistry. Even when Mg2+-proteins share insufficient sequence homology to identify Mg2+-specific sequence motifs, they may still share similarity in the Mg2+-binding site structure. However, no structural motifs characteristic of Mg2+-binding sites have been reported. Thus, our aims are (i) to develop a general method for discovering structural patterns/motifs characteristic of ligand-binding sites, given the 3D protein structures, and (ii) to apply it to Mg2+-proteins sharing <30% sequence identity. Our motif discovery method employs structural alphabet encoding to convert 3D structures to the corresponding 1D structural letter sequences, where the Mg2+-structural motifs are identified as recurring structural patterns.Keywords
This publication has 29 references indexed in Scilit:
- Diversification of function in the haloacid dehalogenase enzyme superfamily: The role of the cap domain in hydrolytic phosphoruscarbon bond cleavageBioorganic Chemistry, 2006
- A substitution matrix for structural alphabet based on structural alignment of homologous proteins and its applicationsProteins-Structure Function and Bioinformatics, 2006
- Protein Block Expert (PBE): a web-based protein structure analysis server using a structural alphabetNucleic Acids Research, 2006
- Recurrent use of evolutionary importance for functional annotation of proteins based on local structural similarityProtein Science, 2006
- Predicting protein function from sequence and structural dataCurrent Opinion in Structural Biology, 2005
- The Protein Data BankActa Crystallographica Section D-Biological Crystallography, 2002
- Prediction of local structure in proteins using a library of sequence-structure motifsJournal of Molecular Biology, 1998
- Satisfying Hydrogen Bonding Potential in ProteinsJournal of Molecular Biology, 1994
- Three-dimensional structure of the free radical protein of ribonucleotide reductaseNature, 1990
- A human retinoic acid receptor which belongs to the family of nuclear receptorsNature, 1987