Construction of BAC libraries derived from the ascidian Ciona intestinalis.
Open Access
- 1 January 2002
- journal article
- Published by Genetics Society of Japan in Genes & Genetic Systems
- Vol. 77 (4), 283-285
- https://doi.org/10.1266/ggs.77.283
Abstract
Large insert genomic bacterial artificial chromosome (BAC) libraries were constructed from a basal chordate, the ascidian Ciona intestinalis. Insert analyses of randomly selected clones indicated that in the first library the mean insert size was 135 kb and predicted a 15-fold coverage of the Ciona genome, and in the second library the mean insert size was 165 kb and predicted a 5-fold coverage of the genome. These first large insert genomic libraries of the ascidian should increase the speed of genomic analyses of basal chordates.Keywords
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