Interaction of tissue transglutaminase with nuclear transport protein importin‐α3
- 5 March 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 446 (1), 35-39
- https://doi.org/10.1016/s0014-5793(99)00018-6
Abstract
Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecipitation to show that tTG interacts with the nuclear transport protein importin-α3. Using electron microscopy we demonstrate that nuclear expression of tTG in a non-small cell lung cancer cell line is induced by retinoic acid (RA). These data suggest that importin-α3 could mediate active nuclear transport of tTG which may be important for the regulation of critical cellular processes.Keywords
This publication has 25 references indexed in Scilit:
- Structure of a Covalently Cross-Linked Form of Core Histones Present in the Starfish SpermBiochemistry, 1997
- Identification and biochemical characterization of an 80 kilodalton GTP-binding/transglutaminase from rabbit liver nucleiBiochemistry, 1995
- The importance of the GTP‐binding protein tissue transglutaminase in the regulation of cell cycle progressionFEBS Letters, 1995
- Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelopeCurrent Biology, 1995
- Biochemical events in naturally occurring forms of cell deathFEBS Letters, 1993
- Differential expression of isopeptide bonds Nε(γ-glutamyl) lysine in benign and malignant human breast lesions: An immunohistochemical studyInternational Journal of Cancer, 1991
- Nuclear protein localizationBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1991
- Activity and gene expression of transglutaminase in guinea pig liver during the postnatal growing phaseFEBS Letters, 1990
- Induction of tissue transglutaminase in human peripheral blood monocytes.The Journal of Experimental Medicine, 1984
- Transglutaminase activity during the differentiation of macrophagesBiochemical and Biophysical Research Communications, 1982