How surface-enhanced chemiluminescence depends on the distance from a corrugated metal film
- 27 November 2006
- journal article
- research article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 89 (22), 223128
- https://doi.org/10.1063/1.2399934
Abstract
Peroxidase labeled streptavidin was immobilized onto the surface of bulk and clusterlike metal films at a distance controlled by a peptide chain with a length between 1.3 and . Luminol chemiluminescence which occurred at peroxidase vicinity depends on the metal nanostructure. When peroxidase is attached on a bulklike film, chemiluminescence increases monotonously with the distance because of a decrease of the light emission quenching by metal. When peroxidase is attached on a clusterlike film, chemiluminescence undergoes a complex variation with the metal/catalyst distance evidencing a competition between the already mentioned quenching process and a nanostructure-induced catalysis enhancement.
Keywords
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