Patterning of Inflorescences and Flowers by the F-Box Protein DOUBLE TOP and the LEAFY Homolog ABERRANT LEAF AND FLOWER of Petunia
- 1 August 2008
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 20 (8), 2033-2048
- https://doi.org/10.1105/tpc.108.060871
Abstract
Angiosperms display a wide variety of inflorescence architectures differing in the positions where flowers or branches arise. The expression of floral meristem identity (FMI) genes determines when and where flowers are formed. In Arabidopsis thaliana, this is regulated via transcription of LEAFY (LFY), which encodes a transcription factor that promotes FMI. We found that this is regulated in petunia (Petunia hybrida) via transcription of a distinct gene, DOUBLE TOP (DOT), a homolog of UNUSUAL FLORAL ORGANS (UFO) from Arabidopsis. Mutation of DOT or its tomato (Solanum lycopersicum) homolog ANANTHA abolishes FMI. Ubiquitous expression of DOT or UFO in petunia causes very early flowering and transforms the inflorescence into a solitary flower and leaves into petals. Ectopic expression of DOT or UFO together with LFY or its homolog ABERRANT LEAF AND FLOWER (ALF) in petunia seedlings activates genes required for identity or outgrowth of organ primordia. DOT interacts physically with ALF, suggesting that it activates ALF by a posttranslational mechanism. Our findings suggest a wider role than previously thought for DOT and UFO in the patterning of flowers and indicate that the different roles of LFY and UFO homologs in the spatiotemporal control of floral identity in distinct species result from their divergent expression patterns.Keywords
This publication has 67 references indexed in Scilit:
- AnArabidopsisF-box protein acts as a transcriptional co-factor to regulate floral developmentDevelopment, 2008
- Floral Initiation and Inflorescence Architecture: A Comparative ViewAnnals of Botany, 2007
- Evolution and Development of Inflorescence ArchitecturesScience, 2007
- PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin PathwayPlant Cell, 2006
- Use of Petunia to unravel plant meristem functioningTrends in Plant Science, 2005
- The F Box Protein Dsg1/Mdm30 Is a Transcriptional Coactivator that Stimulates Gal4 Turnover and Cotranscriptional mRNA ProcessingCell, 2005
- Molecular and Genetic Mechanisms of Floral ControlPlant Cell, 2004
- The Duplicated B-Class Heterodimer Model: Whorl-Specific Effects and Complex Genetic Interactions in Petunia hybrida Flower DevelopmentPlant Cell, 2004
- The COP9 Signalosome Interacts with SCFUFO and Participates in Arabidopsis Flower DevelopmentPlant Cell, 2003
- Engineered GFP as a vital reporter in plantsCurrent Biology, 1996