Determination of a 21548 bp nucleotide sequence around the 24 region of the Bacillus subtilis chromosome

Abstract

A 21 548 bp nucleotide sequence around the 24" region of the Bacillus subfilis chromosome, located 306 kb downstream from the zero point of the physical map, was determined. Twenty-one putative ORFs were identified: two ORFs (Orfl and Orf20) were consistent with the nucleotide and amino acid sequence of B. subfilis OrfJ, whose function is not known, and Pcp, respectively; four were found to display significant similarities to known proteins in data banks, i .e. 5-keto-3-deoxyg lucarate de hydratase (Orf2), aldehyde de h ydrogenase (Orf3) and two glucarate dehydratases (Orf4 and 5); three had considerable similarity to the sensor-regulator proteins of bacterial two-component signal transduction systems (Orfl, 11 and 12); two had considerably high levels of similarity to parts of known proteins (Orfl3 and 19); and five showed low levels of similarity to known proteins in the data banks. The remaining six ORFs showed no similarity to known proteins.