The Cloning of PIG-A, a Component in the Early Step of GPI-Anchor Biosynthesis
- 26 February 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 259 (5099), 1318-1320
- https://doi.org/10.1126/science.7680492
Abstract
The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.Keywords
This publication has 24 references indexed in Scilit:
- Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surfaceCell, 1992
- Topography of glycosylation reactions in the endoplasmic reticulumTrends in Biochemical Sciences, 1992
- Phosphatidylinositol membrane anchors and T-cell activationImmunology Today, 1991
- Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchorThe Journal of Membrane Biology, 1990
- The glycosyl-phosphatidylinositol anchor of membrane proteinsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1989
- Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoproteinNature, 1988
- Glycosyl-Phosphatidylinositol Moiety That Anchors Trypanosoma brucei Variant Surface Glycoprotein to the MembraneScience, 1988
- High lateral mobility of endogenous and transfected alkaline phosphatase: a phosphatidylinositol-anchored membrane protein.The Journal of cell biology, 1987
- Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria.The Journal of Experimental Medicine, 1985
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967