TYROSINASE INHERITANCE IN STREPTOMYCES SCABIES II
Open Access
- 1 June 1964
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 87 (6), 1287-1294
- https://doi.org/10.1128/jb.87.6.1287-1294.1964
Abstract
Gregory, Kenneth F. (Ontario Agricultural College, Guelph, Ontario, Canada), and Jay C. C. Huang. Tyrosinase inheritance in Streptomyces scabies. II. Induction of tyrosinase deficiency by acridine dyes. J. Bacteriol. 87:1287–1294. 1964.—Growth in minimal medium containing 1 μg of acriflavine per ml resulted in a large increase (up to 62%) in the frequency of tyrosinase-deficient (tye−) mutants in all of ten strains of Streptomyces scabies and eight unidentified streptomycetes studied. This increased frequency did not result from the selection of preformed mutants, since tye− clones were usually inhibited by lower concentrations of acriflavine than were tyrosinase-producing (tye+) clones, and no significant difference in mycelial yields occurred between the two types growing in a 1 μg/ml concentration of the dye. The mutations induced by X rays and acriflavine were either allelic or closely linked. This tye− phenotype was not caused by the production of an enzyme inhibitor, lack of a cofactor, or defect in the conversion of a protyrosinase to tyrosinase. Tye− mutants formed no detectable tyrosinase under a variety of conditions, including the presence of possible inducers. Mutants were able to oxidize glucose and succinate. The S. scabies tyrosinase was heat-labile (half-life at 59 C = 1.6 min) and not particle-bound. We conclude that acriflavine induces the loss of, or alteration in, a structural gene for tyrosinase production present as an extrachromosomal factor.Keywords
This publication has 26 references indexed in Scilit:
- COPPER, TYROSINASE, AND THE KINETIC STABILITY OF OXYGENAnnals of the New York Academy of Sciences, 1963
- Preliminary Studies on the Basis of Cytoplasmic Inheritance in AmœbæNature, 1962
- Apparent Cytoplasmic Inheritance of Tyrosinase Competence in Streptomyces ScabiesNature, 1961
- THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLIProceedings of the National Academy of Sciences of the United States of America, 1960
- Cytoplasmic InheritanceNature, 1959
- The effect of the inhibition of protein synthesis on the establishment of lysogenyVirology, 1957
- PATHOGENICITY OF TYROSINASE-DEFICIENT MUTANTS OF STREPTOMYCES SCABIESCanadian Journal of Microbiology, 1956
- ENZYME LOCALIZATION IN Azotobacter VinelandiiProceedings of the National Academy of Sciences of the United States of America, 1955
- Measurement of the Cresolase Activity of TyrosinaseJournal of the American Chemical Society, 1947
- A New Method for the Measurement of Tyrosinase Catecholase Activity. II. Catecholase Activity Based on the Initial Reaction VelocityJournal of the American Chemical Society, 1944