Construction of lac fusions to the inducible arginine‐and lysine decarboxylase genes of Escherichia coli K12
- 30 April 1989
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 3 (5), 609-620
- https://doi.org/10.1111/j.1365-2958.1989.tb00208.x
Abstract
The induction of several amino acid decarboxylases under anaerobic conditions at low pH has been known for many years, but the mechanism associated with this type of regulation has not been elucidated. To study the regulation of the biodegradative arginine and lysine decarboxylases of Escherichia coli K12, Mudlac fusions to these genes were isolated. Mudlac fusion strains deficient for lysine decarboxylase or arginine decarboxylase were identified using decarboxylase indicator media and analysed for their regulation of β-galactosidase expression. The position of the Mud-lac fusion in lysine decarboxylase-deficient strains has been mapped to the cadA gene at 93.7 minutes, while the Mudlac fusions exhibiting a deficiency in the inducible arginine decarboxylase have been mapped to 93.4 minutes.This publication has 56 references indexed in Scilit:
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