Immuno-Electrophoretic Study of the Human Perilymph

Abstract

The authors have isolated by immuno-electrophoretic study thirteen different protein fractions in the normal human perilymph. Two antisera were used (generally simultaneously) on the plate of the Grabar apparatus: a horse, anti-human serum, routinely prepared and tested by the Pasteur Institute of Paris (Grabar & Burtin), and a rabbit, anti-human, spinalfluid serum, specially prepared and stocked after liophylization by the authors for the present study. The comparison of the present results with those reported by various authors dealing not with the perilymph but with the electrophoretic fractions of the human, normal spinal fluid, does not seem to support the theory stating that the perilymph is a mere dialysate from the spinal fluid. Such studies may nevertheless in the future be of interest in various experimental physio-pathological states of the perilymph in laboratory animals.