Immunohistochemical localization and distribution of proliferating cell nuclear antigen in the rabbit mandibular condyle following experimental induction of anterior disk displacement.
- 1 April 2002
- journal article
- other
- Published by Informa UK Limited in Cranio®
- Vol. 20 (2), 111-115
- https://doi.org/10.1080/08869634.2002.11746199
Abstract
The purpose of the present study was to identify proliferating cells in control versus experimental condyles two weeks following experimental induction of anterior disk displacement (ADD) in the rabbit craniomandibular joint (CMJ). The right joint of 15 rabbits was exposed surgically and all diskal attachments were severed except for the posterior attachment. The disk was then repositioned anteriorly and sutured to the zygomatic arch. The left joint served as a sham-operated control. Ten additional joints were used as nonoperated controls. Mandibular condyles were excised two weeks following surgery and processed for proliferating cell nuclear antigen (PCNA) immunostaining. In control and sham operated condyles, PCNA was localized in the nuclei of chondroblasts of the reserve cell layer, chondrocytes of the upper hypertrophic layer and bone marrow cells of the subchondral bone. In contrast to control joints, the PCNA positive cells of the experimental joints were located throughout the osteoarthritic condylar cartilage. In addition, the percentage of PCNA positive cells of the osteoarthritic condylar cartilage was statistically significantly higher when compared to the control group, p < 0.05. It was concluded that surgical induction of ADD in the rabbit CMJ leads to an increase in mitosis of chondrocytes, which lead to cell proliferation and subsequent hyperplasia of the condylar cartilage.Keywords
This publication has 15 references indexed in Scilit:
- Enlargement of the rabbit mandibular condyle after experimental induction of anterior disc displacementJournal of Oral and Maxillofacial Surgery, 1995
- Histopathological changes in rabbit craniomandibular joint associated with experimentally induced anterior disk displacement (ADD)Journal of Oral Pathology & Medicine, 1994
- Serial variation in histological character of articular soft tissue in young human adult temporomandibular joint condylesArchives of Oral Biology, 1993
- The Relationship of Undifferentiated Mesenchymal Cells to TMJ Articular Tissue ThicknessJournal of Dental Research, 1992
- Effect of condylotomy on DNA synthesis in cells of the mandibular condylar cartilage in the ratArchives of Oral Biology, 1987
- [3H]thymidine uptake in cells of rat condylar cartilageJournal of Anatomy, 1983
- Histological analysis of the growth of the mandibular condyle in the rhesus monkey (Macaca mulatta)Journal of Anatomy, 1978
- Chondrocyte Mitosis in the Articular Cartilage of Femoral Heads with Various DiseasesActa Orthopaedica, 1975
- Further evidence for the vitality of chondrocytes in the mandibular condyle as revealed by35S-sulfate autoradiographyThe Anatomical Record, 1972
- Vitality of chondrocytes in the mandibular condyle as revealed by collagen formation. An autoradiographic study with 3H‐prolineJournal of Anatomy, 1972