THE APPEARANCE AND STRUCTURE OF INTERCELLULAR CONNECTIONS DURING THE ONTOGENY OF THE RABBIT OVARIAN FOLLICLE WITH PARTICULAR REFERENCE TO GAP JUNCTIONS
Open Access
- 1 October 1974
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 63 (1), 234-250
- https://doi.org/10.1083/jcb.63.1.234
Abstract
Lanthanum tracer and freeze-fracture electron microscope techniques were used to study junctional complexes between granulosa cells during the differentiation of the rabbit ovarian follicle. For convenience we refer to cells encompassing the oocyte, before antrum and gap junction formation, as follicle cells. After the appearance of an antrum and gap junctions we call the cells granulosa cells. Maculae adherentes are found at the interfaces of oocyte-follicle-granulosa cells throughout folliculogenesis. Gap junctions are first detected in follicles when the antrum appears. In early antral follicles typical large gap junctions are randomly distributed between granulosa cells. In freeze-fracture replicas, they are characterized by polygonally packed 90-A particles arranged in rows separated by nonparticulate A-face membrane. A particle-sparse zone surrounds gap junctions and is frequently occupied by small particle aggregates of closely packed intramembranous particles. The gap junctions of granulosa cells appear to increase in size with further differentiation of the follicle. The granulosa cells of large Graafian follicles are adjoined by small and large gap junctions; annular gap junctions are also present. The large gap junctions are rarely surrounded by a particle-free zone on their A-faces, but are further distinguished by particle rows displaying a higher degree of organization.Keywords
This publication has 33 references indexed in Scilit:
- THE ULTRASTRUCTURE OF THE NEXUSThe Journal of cell biology, 1970
- Effects of Stage of the Menstrual Cycle and Gonadotrophins on Luteinization of Rhesus Monkey Granulosa Cells in Culture1Endocrinology, 1970
- MEMBRANE SPLITTING IN FREEZE-ETCHINGThe Journal of cell biology, 1970
- JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN THE VERTEBRATE BRAINThe Journal of cell biology, 1969
- Ultrastructural studies on the preovulatory follicle in the mouse ovaryCell and tissue research, 1969
- The fate of desmosomes in trypsinized tissueJournal of Experimental Zoology, 1968
- A Modified Method for Lead Staining of Thin SectionsJournal of Electron Microscopy, 1968
- Ultrastructural studies of the bovine Graafian Follicle and corpus luteumCell and tissue research, 1968
- HEXAGONAL ARRAY OF SUBUNITS IN INTERCELLULAR JUNCTIONS OF THE MOUSE HEART AND LIVERThe Journal of cell biology, 1967
- Fracture faces of frozen membranes.Proceedings of the National Academy of Sciences of the United States of America, 1966