Mechanism of action of cytochalasin: evidence that it binds to actin filament ends.
Open Access
- 1 March 1981
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 88 (3), 487-491
- https://doi.org/10.1083/jcb.88.3.487
Abstract
To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to make filament ends (Brown and Spudich, 1979, J. Cell Biol. 80:499-504) and then reacted with [3H]cytochalasin B. We have found that cytochalasin B binds to beads in the presence of actin, and that the number of cytochalasin B binding sites can be varied as a function of the number of filament ends independent of the total actin concentration by varying the bead concentration.This publication has 15 references indexed in Scilit:
- ATP-driven steady-state exchange of monomeric and filamentous actin from Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America, 1980
- Mechanism of action of cytochalasin B on actinCell, 1980
- Spectrin/actin complex isolated from sheep erythrocytes accelerates actin polymerization by simple nucleation. Evidence for oligomeric actin in the erythrocyte cytoskeleton.Journal of Biological Chemistry, 1980
- Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin.Journal of Biological Chemistry, 1980
- Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation.The Journal of cell biology, 1980
- Cytochalasin B and the structure of actin gelsJournal of Molecular Biology, 1979
- Substoichiometric concentrations of cytochalasin D inhibit actin polymerization. Additional evidence for an F-actin treadmill.Journal of Biological Chemistry, 1979
- Nucleation of polar actin filament assembly by a positively charged surface.The Journal of cell biology, 1979
- Biochemical and structural characterization of actin from Dictyostelium discoideum.Journal of Biological Chemistry, 1978
- [45] Reductive alkylation of amino groupsMethods in enzymology, 1977