RELEASE OF PROSTAGLANDINS FROM THE RABBIT PERFUSED KIDNEY: EFFECTS OF VASOCONSTRICTORS

Abstract

1 The rat stomach strip was used to assay prostaglandin E₂-like material released by a rabbit isolated kidney perfused with Krebs solution. 2 Doses of noradrenaline and angiotensin II producing similar vasoconstrictor effects released equivalent amounts of prostaglandins from the kidney. 3 8-Leu-angiotensin II, a specific inhibitor of the natural octapeptide, blocked the action of angiotensin II on perfusion pressure and the release of prostaglandins, while the action of noradrenaline on both parameters was unaffected. 4 Indomethacin, a specific inhibitor of prostaglandin biosynthesis, blocked the effects of both vasoconstrictors on prostaglandin release while their action on perfusion pressure was significantly enhanced. 5 In the kidney effluent, amounts of prostaglandin E₂-like material increased linearly with the rise in perfusion pressure induced by increasing doses of angiotensin II. These results indicate that prostaglandin output from the isolated kidney follows the rise in perfusion pressure.