SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER

Abstract

Hybridization of RNA with DNA from a transducing phage carrying the lac genetic region and chromatographic fractionation of RNA were employed to detect the specific lac message RNA in E. coli. The experiments demonstrate the occurrence in induced cells of increased amounts of RNA messages complementary to the lac region. This RNA can be identified chromatographically and distinguished from other E. coli messages which possess homology for some portions of the phage genome. The data are consistent with the conclusion that the presence of inducer leads to an increased rate of transcription from the lac region.