In utero phenotyping of mouse embryonic vasculature with MRI
Open Access
- 16 May 2011
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 67 (1), 251-257
- https://doi.org/10.1002/mrm.22991
Abstract
The vasculature is the earliest developing organ in mammals and its proper formation is critical for embryonic survival. MRI approaches have been used previously to analyze complex three‐dimensional vascular patterns and defects in fixed mouse embryos. Extending vascular imaging to an in utero setting with potential for longitudinal studies would enable dynamic analysis of the vasculature in normal and genetically engineered mouse embryos, in vivo. In this study, we employed an in utero MRI approach that corrects for motion, using a combination of interleaved gated acquisition and serial coregistration of rapidly acquired three‐dimensional images. We tested the potential of this method by acquiring and analyzing images from wildtype and Gli2 mutant embryos, demonstrating a number of Gli2 phenotypes in the brain and cerebral vasculature. These results show that in utero MRI can be used for in vivo phenotype analysis of a variety of mutant mouse embryos. Magn Reson Med, 2011.Keywords
This publication has 35 references indexed in Scilit:
- Three‐dimensional micro‐MRI analysis of cerebral artery development in mouse embryosMagnetic Resonance in Medicine, 2009
- Three‐dimensional, in vivo MRI with self‐gating and image coregistration in the mouseMagnetic Resonance in Medicine, 2009
- A Membrane Associated mCherry Fluorescent Reporter Line for Studying Vascular Remodeling and Cardiac Function During Murine Embryonic DevelopmentThe Anatomical Record, 2009
- Mn enhancement and respiratory gating for in utero MRI of the embryonic mouse central nervous systemMagnetic Resonance in Medicine, 2008
- Hedgehog signalling in vascular developmentAngiogenesis, 2008
- MRI in mouse developmental biologyNMR in Biomedicine, 2007
- Contrast‐enhanced MRI of right ventricular abnormalities in Cx43 mutant mouse embryosNMR in Biomedicine, 2007
- Spatial pattern of sonic hedgehog signaling throughGligenes during cerebellum developmentDevelopment, 2004
- High‐resolution imaging of normal anatomy, and neural and adrenal malformations in mouse embryos using magnetic resonance microscopyJournal of Anatomy, 2003
- Alteration in connexin 43 gap junction gene dosage impairs conotruncal heart developmentDevelopmental Biology, 1998