A requirement for MAP kinase in the assembly and maintenance of the mitotic spindle
Open Access
- 23 June 2003
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 161 (6), 1021-1028
- https://doi.org/10.1083/jcb.200304144
Abstract
Circumstantial evidence has suggested the possibility of microtubule-associated protein (MAP) kinase's involvement in spindle regulation. To test this directly, we asked whether MAP kinase was required for spindle assembly in Xenopus egg extracts. Either the inhibition or the depletion of endogenous p42 MAP kinase resulted in defective spindle structures resembling asters or half-spindles. Likewise, an increase in the length and polymerization of microtubules was measured in aster assays suggesting a role for MAP kinase in regulating microtubule dynamics. Consistent with this, treatment of extracts with either a specific MAP kinase kinase inhibitor or a MAP kinase phosphatase resulted in the rapid disassembly of bipolar spindles into large asters. Finally, we report that mitotic progression in the absence of MAP kinase signaling led to multiple spindle abnormalities in NIH 3T3 cells. We therefore propose that MAP kinase is a key regulator of the mitotic spindle.Keywords
This publication has 25 references indexed in Scilit:
- Distinct Cell Cycle Timing Requirements for Extracellular Signal-Regulated Kinase and Phosphoinositide 3-Kinase Signaling Pathways in Somatic Cell MitosisMolecular and Cellular Biology, 2002
- Meiotic spindle stability depends on MAPK-interacting and spindle-stabilizing protein (MISS), a new MAPK substrateThe Journal of cell biology, 2002
- The Mitotic Spindle: A Self-Made MachineScience, 2001
- Specificity and mechanism of action of some commonly used protein kinase inhibitorsBiochemical Journal, 2000
- Cloning and Characterization of Xenopus Rsk2, the Predominant p90 Rsk Isozyme in Oocytes and EggsPublished by Elsevier BV ,2000
- The Protein Kinase p90 Rsk as an Essential Mediator of Cytostatic Factor ActivityScience, 1999
- Chapter 20 The Use of Xenopus Egg Extracts to Study Mitotic Spindle Assembly and Function in VitroMethods in cell biology, 1998
- In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinaseNature, 1991
- Cyclin activation of p34cdc2Cell, 1990
- Tubulin dynamics in cultured mammalian cells.The Journal of cell biology, 1984