Elucidation of Carotenoid Patterns in Citrus Products by Means of Comprehensive Normal-Phase × Reversed-Phase Liquid Chromatography
- 19 October 2006
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (22), 7743-7750
- https://doi.org/10.1021/ac061290q
Abstract
A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids were separated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silica column in the first dimension (first D), reversed phase with a monolithic C18 column in the second dimension (second D), and a 10-port switching valve as an interface. An on-line photodiode array detector was used in order to obtain absorption spectra. Peak identification was obtained by combining retention data with the UV−visible spectra.Keywords
This publication has 19 references indexed in Scilit:
- Considerations on the possibilities and limitations of comprehensive normal phase–reversed phase liquid chromatography (NPLC×RPLC)Journal of Separation Science, 2006
- Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysisJournal of Chromatography A, 2005
- Comprehensive two-dimensional chromatography in food analysisJournal of Chromatography A, 2004
- Improved Normal-Phase High-Performance Liquid Chromatography Procedure for the Determination of Carotenoids in CerealsJournal of Agricultural and Food Chemistry, 2004
- Comprehensive Two-Dimensional Normal-Phase (Adsorption)−Reversed-Phase Liquid ChromatographyAnalytical Chemistry, 2004
- Identification and Quantification of Carotenoids Including Geometrical Isomers in Fruit and Vegetable Juices by Liquid Chromatography with Ultraviolet−Diode Array DetectionJournal of Agricultural and Food Chemistry, 2004
- Distribution of Lutein, Zeaxanthin, and Related Geometrical Isomers in Fruit, Vegetables, Wheat, and Pasta ProductsJournal of Agricultural and Food Chemistry, 2003
- Determination of the geographical origin of valencia orange juice using carotenoid liquid chromatographic profilesJournal of Chromatography A, 1999
- HPLC analysis of carotenoids in orange juiceFood Chemistry, 1999
- Application of Diode Array Detection with a C-30 Reversed Phase Column for the Separation and Identification of Saponified Orange Juice CarotenoidsJournal of Agricultural and Food Chemistry, 1996