Imaging of protein movement induced by chromosomal breakage: tiny ‘local’ lesions pose great ‘global’ challenges
- 30 June 2005
- journal article
- review article
- Published by Springer Science and Business Media LLC in Chromosoma
- Vol. 114 (3), 146-154
- https://doi.org/10.1007/s00412-005-0011-y
Abstract
Interruption of chromosomal integrity by DNA double-strand breaks (DSBs) causes a major threat to genomic stability. Despite tremendous progress in understanding the genetic and biochemical aspects of DSB-induced genome surveillance and repair mechanisms, little is known about organization of these molecular pathways in space and time. Here, we outline the key spatio-temporal problems associated with DSBs and focus on the imaging approaches to visualize the dynamics of DSB-induced responses in mammalian cells. We delineate benefits and limitations of these assays and highlight the key recent discoveries where live microscopy provided unprecedented insights into how cells defend themselves against genome-destabilizing effects of DNA damage.Keywords
This publication has 61 references indexed in Scilit:
- Concepts in nuclear architectureBioEssays, 2005
- DNA Damage Response Pathway Uses Histone Modification to Assemble a Double-Strand Break-Specific Cohesin DomainMolecular Cell, 2004
- Checking on DNA damage in S phaseNature Reviews Molecular Cell Biology, 2004
- Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaksNature, 2003
- Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centreNature, 2003
- DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociationNature, 2003
- DNA Damage during Mitosis in Human Cells Delays the Metaphase/Anaphase Transition via the Spindle-Assembly CheckpointCurrent Biology, 2002
- Translating the Histone CodeScience, 2001
- Chromosomal microdissection by laser: A cytochemical and functional analysisExperimental Cell Research, 1971
- Effects of laser micro-irradiation on chromosomesExperimental Cell Research, 1969