Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies
- 1 March 1986
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 90 (1-2), 97-110
- https://doi.org/10.1007/bf01314148
Abstract
Twelve monoclonal antibodies to the F protein of a velogenic strain of Newcastle disease virus (NDV) were established. Each of these antibodies inhibited virus-induced plaque formation in BHK-21 cells. Seven antibodies neutralized viral infectivity in eggs; thus, antigenic variants could be selected with these antibodies and used for antigenic mapping. Based on the reactivity of the antigenic variants with the antibodies used in selection, 4 distinct antigenic sites (I–IV) were defined on the F protein molecule. In competitive binding assay, sites II and III were found to be spatially close to each other. Each antibody to sites I, II and III inhibited both virus-induced hemolysis of chicken erythrocytes and syncytium formation of BHK-21 cells. On the other hand, some of the antibodies to site IV selectively inhibited either hemolysis or cell fusion. This finding may indicate that the fusion of the viral envelope with erythrocytes and host cell membrane is modulated through different ways. Comparative analysis of different NDV strains using monoclonal antibodies to each of the different antigenic sites showed that the antigenicity of the F protein is highly conserved.This publication has 55 references indexed in Scilit:
- Sequence Determination of the Sendai Virus Fusion Protein GeneJournal of General Virology, 1985
- Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodiesArchiv für die gesamte Virusforschung, 1983
- Monoclonal antibodies against a paramyxovirus isolated from Japanese Sparrow-Hawks(Accipiter virugatus gularis)Archiv für die gesamte Virusforschung, 1983
- Inhibition of virus-induced hemolysis with monoclonal antibodies to different antigenic areas on the hemagglutinin molecule of A/seal/Massachusetts/1/80 (H7N7) influenza virusArchiv für die gesamte Virusforschung, 1983
- Involvement of Glycolipids in Myxovirus-induced Membrane Fusion (Haemolysis)Journal of General Virology, 1983
- The structural polypeptides of avian paramyxovirusesArchiv für die gesamte Virusforschung, 1981
- Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the Fl or HA2 viral polypeptidesVirology, 1980
- Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection.The Journal of Experimental Medicine, 1980
- The Role of Viral Glycoproteins in Adsorption, Penetration, and Pathogenicity of VirusesClinical Infectious Diseases, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979