Cryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora): importance of water content and cooling rate
- 1 March 1998
- journal article
- research article
- Published by Cambridge University Press (CUP) in Seed Science Research
- Vol. 8 (1), 9-15
- https://doi.org/10.1017/s096025850000386x
Abstract
In the range of water contents studied (0.1–0.4 g H2O g dw−1),Coffea arabicaseeds were less sensitive to desiccation thanC. costatifructa, C. racemosaandC. sessilifloraseeds. At 0.20 g H2O g dw−1, 53% ofC. arabicaseeds germinated after direct immersion in LN (rapid cooling, 200°C min−1), but none of them developed into normal seedlings. By contrast, inC. costatifructa, C. racemosaandC. sessiliflora, when seeds were dehydrated to the optimal water content (0.19, 0.28 and 0.31 g H2O g dw−1, respectively), the percentages of seeds which developed into normal seedlings after LN exposure were 26, 78 and 31% of the desiccation control, respectively. Normal seedlings could be recovered from cryopreservedC. arabicaseeds only if they were desiccated to 0.20 g H2O g dw−1and precooled slowly to −50°C prior to immersion in LN. Precooling seeds at 2°C min−1allowed 25% of seeds to develop into normal seedlings. The thawing rate had no effect on the survival of cryopreservedC. arabicaseeds. In all cryopreservation experiments, the total germination did not reflect the percentage of seeds which developed into normal seedlings. Examination of excised embryos indicated a partial explanation of this difference since only the shoot apex was destroyed in abnormal embryos, whereas the hypocotyl and radicle were normal.Keywords
This publication has 12 references indexed in Scilit:
- Variability in storage response within a coffee ( Coffea spp.) core collection under slow growth conditionsPlant Cell Reports, 1997
- Cryopreservation of Recalcitrant SeedsPublished by Springer Science and Business Media LLC ,1995
- The effects of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L.Seed Science Research, 1991
- An Intermediate Category of Seed Storage Behaviour?II. EFFECTS OF PROVENANCE, IMMATURITY, AND IMBIBITION ON DESICCATION-TOLERANCE IN COFFEEJournal of Experimental Botany, 1991
- An Intermediate Category of Seed Storage Behaviour?Journal of Experimental Botany, 1990
- Effects of Cooling Rate on Seeds Exposed to Liquid Nitrogen TemperaturesPlant Physiology, 1989
- Desiccation and storage studies on Dipterocarpm seedsAnnals of Applied Biology, 1987
- Survival of Sesame Seeds at the Temperature (−196°C) of Liquid Nitrogen1Crop Science, 1987
- Desiccation studies in relation to the storage of Araucaria seedAnnals of Applied Biology, 1984
- Significance tests for multiple comparison of proportions, variances, and other statistics.Psychological Bulletin, 1960