Effects of Seedling Age on Colonization Patterns of Citrus limon Plants by Endophytic Beauveria bassiana and Metarhizium anisopliae and Their Influence on Seedlings Growth
Open Access
- 24 February 2020
- journal article
- research article
- Published by MDPI AG in Journal of Fungi
- Vol. 6 (1), 29
- https://doi.org/10.3390/jof6010029
Abstract
The inoculation methods, the fungal strains, and several other factors are known to influent the success of fungal entomopathogens colonization in plants. The physiological status of the plant could also be another determinant. In the present study, the ability of three strains of Beauveria bassiana and one strain of Metarhizium anisopliae to successfully colonize Citrus limon plants and the influence of seedling age on endophytic colonization success was examined. Three, 4, and 6 months old seedlings were inoculated with 10 mL of 1 × 108 conidial·mL−1 suspensions of each of the four fungal strains via foliar spraying. All fungal strains successfully colonized citrus seedlings and were sustained up to 2 months in colonized plants irrespective of the seedling age, with differences in the mean percentage colonization recorded at various post-inoculation periods among the fungal strains. The highest percent endophytic fungi recovery rate was recorded in the 3 months old seedlings, where fungal mycelia of inoculated fungi were successfully re-isolated from 65.6% of the untreated newly developed leaf and stem tissues. One strain of B. bassiana, BB Fafu-12, significantly improved seedling height and leaf number. The study demonstrates the influence of seedling age on B. bassiana and M. anisopliae successful colonization in the citrus plant.Keywords
Funding Information
- Fujian Agriculture and Forestry University (CXZX2017211)
This publication has 51 references indexed in Scilit:
- Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological ControlJournal of Visualized Experiments, 2013
- Endophytic colonization of Vicia faba and Phaseolus vulgaris (Fabaceae) by fungal pathogens and their effects on the life-history parameters of Liriomyza huidobrensis (Diptera: Agromyzidae)Fungal Ecology, 2013
- Persistence of Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte following inoculation of radiata pine seed and seedlingsBiological Control, 2012
- Systemic acropedal influence of endophyte seed treatment on Acyrthosiphon pisum and Aphis fabae offspring development and reproductive fitnessBiological Control, 2012
- Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassianaBioControl, 2009
- Metarhizium anisopliae Seed Treatment Increases Yield of Field Corn When Applied for Wireworm ControlAgronomy Journal, 2007
- Establishment of the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte in cocoa seedlings (Theobroma cacao)Mycologia, 2005
- Emerging Perspectives on the Ecological Roles of Endophytic Fungi in Tropical PlantsPublished by Informa UK Limited ,2005
- Endophyticbeauveria bassiana(balsamo) vuillemin in corn: The influence of the plant growth stage andostrinia nubilalis(hübner)Biocontrol Science and Technology, 1992
- Fungal endophytes in Salicornia perennisTransactions of the British Mycological Society, 1986