DNA flow cytometry of human breast carcinomas and its relationship to transferrin and epidermal growth factor receptors
- 1 September 1986
- journal article
- research article
- Published by Wiley in The Journal of Pathology
- Vol. 150 (1), 37-42
- https://doi.org/10.1002/path.1711500107
Abstract
The expression of transferrin and epidermal growth factor receptors in breast carcinomas, as detected by immunohistochemistry, has been compared with DNA ploidy and S-phase content of the same tumours as determined by DNA flow cytometry of fixed, paraffin embedded tissue, and with tumour differentiation. Good correlations have been found between DNA ploidy, S-phase content and differentiation. The expression of transferrin receptor appears to relate to DNA ploidy, but only to a limited extent to S-phase content, indicating that in breast carcinomas immunoreactive transferrin receptor may not be directly related to proliferation. The presence of epidermal growth factor receptor shows a close correlation with high S-phase content, but only a lesser association with DNA ploidy, and no correlation with tumour differentiation. DNA flow cytometry provides a more reliable method of assessing the relationship of such growth factor receptors in breast carcinomas to cell proliferation, than that obtained from more subjective tumour grading criteria.Keywords
This publication has 25 references indexed in Scilit:
- DNA flow cytometry of histological material from human gastric cancerThe Journal of Pathology, 1986
- Transferrin receptor expression in non-malignant and malignant human breast tissueThe Journal of Pathology, 1986
- Presence of epidermal growth factor receptor as an indicator of poor prognosis in patients with breast cancer.Journal of Clinical Pathology, 1985
- Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma.Journal of Clinical Pathology, 1985
- MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGFBiochemical and Biophysical Research Communications, 1985
- The transferrin receptorTrends in Biochemical Sciences, 1982
- Ploidy, proliferative activity and estrogen receptor content in human breast cancerCytometry, 1982
- Epidermal growth factor requirement for development of cultured mammary glandNature, 1980
- Analysis of reproducibility of subjective grading systems for breast carcinoma.Journal of Clinical Pathology, 1979
- Transferrin and iron uptake by human cells in cultureExperimental Cell Research, 1974