High-resolution 31P nuclear magnetic resonance study of rat liver mitochondria.
- 1 April 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (4), 1796-1800
- https://doi.org/10.1073/pnas.75.4.1796
Abstract
Intact mitochondria were studied by high-resolution 31P NMR. Observable internal phosphate compounds included Pi, ADP and ATP. The internal pH was determined by the chemical shift of the internal Pi, the pK2 (6.7) of which was measured in uncoupled mitochondria. The observed equilibrium relation between the internal and the external Pi was consistent with the exchange equilibrium through the H2PO4-/OH- carrier. The internal ATP and ADP were essentially Mg2+ bound and their resonances were distinguishable from those of the external ATP and ADP by the chemical shift differences due to the Mg2+ concentration gradient and .DELTA.pH. Oxidative phosphorylation was followed by the separate resonances of Pi and adenine nucleotides both internal and external to mitochondria. From these resonances the internal and external phosphate potentials could be estimated.Keywords
This publication has 13 references indexed in Scilit:
- 31P Nuclear magnetic resonance studies of cell membranes labelled with phosphonium phosphatidylcholineFEBS Letters, 1977
- Active proton uptake by chromaffin granules: observation by amine distribution and phosphorus-31 nuclear magnetic resonance techniquesBiochemistry, 1977
- High-resolution 31P nuclear magnetic resonance studies of metabolism in aerobic Escherichia coli cells.Proceedings of the National Academy of Sciences, 1977
- Phosphorus nuclear magnetic resonance studies on normoxic and ischemic cardiac tissue.Proceedings of the National Academy of Sciences, 1976
- Observation of tissue metabolites using 31P nuclear magnetic resonanceNature, 1974
- Phosphate transport in rat liver mitochondria. Kinetics and energy requirements.1974
- The mechanism of energy-dependent ion transport in mitochondriaThe Journal of Membrane Biology, 1973
- The value of ΔG° for the hydrolysis of ATPBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- The osmotic nature of the ion-induced swelling of rat-liver mitochondriaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1969