A Simple Method for Determination of Corneal Epithelial Permeability in Humans
- 1 January 1987
- journal article
- Published by Informa UK Limited in Current Eye Research
- Vol. 6 (11), 1327-1334
- https://doi.org/10.3109/02713688708997558
Abstract
A simple method for the determination of human corneal epithelial permeability to fluorescein is presented. The method consists of applying a 1% sodium fluorescein solution to the cornea for 8 minutes by means of an eye bath, rinsing the eye, and measuring corneal fluorescence by fluorophotometry. The permeability value is calculated from the corneal fluorescein concentration immediately after the bathing period. Mean permeability value determined in 86 eyes of 46 volunteers aged 15 to 67 years (mean 30.9 years) was 0.038 nm/s +/- 0.017 SD. No significant correlation with age was found (corr. coeff. = 0.17, P = 0.28). The reproducibility was within 10%.Keywords
This publication has 11 references indexed in Scilit:
- The rate of diffusion of fluorophores through the corneal epithelium and stromaExperimental Eye Research, 1987
- A reevaluation of corneal endothelial permeability to fluoresceinExperimental Eye Research, 1984
- A Fresh Look at IontophoresisAmerican Journal of Ophthalmology, 1984
- THE THICKNESS OF THE HUMAN CORNEA AS DETERMINED BY A SPECULAR METHODActa Ophthalmologica, 1984
- The diffusion of fluorescein in the stroma of rabbit corneaExperimental Eye Research, 1983
- Basal Precorneal Tear Turnover in the Human EyeAmerican Journal of Ophthalmology, 1980
- The influence of non-ionic detergents and other surfactants on human corneal permeabilityExperimental Eye Research, 1971
- The effect of viscosity of the vehicle on the penetration of fluorescein into the human eyeExperimental Eye Research, 1971
- New methods of measuring the rate of aqueous flow in man with fluoresceinExperimental Eye Research, 1966
- The Movement of Fluorescein and Water in the Cornea*American Journal of Ophthalmology, 1960