Mediterranean Mussel Gene Expression Profile Induced by Okadaic Acid Exposure
- 29 September 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 44 (21), 8276-8283
- https://doi.org/10.1021/es102213f
Abstract
Seasonal seawater temperature increases define optimal growth conditions for Dinoflagellate species which can reach high concentrations in water column and also in filter-feeding organisms like Mytilus galloprovincialis. Commonly produced by Dinophysis and Prorocentrum spp., okadaic acid (OA) and its analogues are responsible for the Diarrheic Shellfish Poisoning (DSP) syndrome in humans. Closure of shellfishing grounds is therefore recommended by the EU when DSP toxin levels in shellfish exceed 16 μg OA 100 g−1 flesh. Despite not being responsible for casualties either in humans or mussels, DSP outbreaks are considered natural events causing health and economic issues due to the frequency of their occurrence. Since gene expression studies offer a wide range of different solutions, we used a mussel cDNA microarray to evaluate gene expression changes in the digestive gland of mussels fed for five weeks with OA-contaminated nutrient. Among the differentially expressed genes we observed a general up-regulation of transcripts coding for stress proteins, proteins involved in cellular synthesis, and a few not annotated proteins. Overall, at the first time point analyzed we identified 58 candidate transcripts for OA-induced stress in mussels, half of which have unknown function. In this paper we present the first gene expression analysis performed on Mediterranean mussels exposed to okadaic acid. The characterization of these transcripts could be useful for the identification of an early physiological response to OA exposure.Keywords
This publication has 34 references indexed in Scilit:
- Intra-amygdalar okadaic acid enhances conditioned taste aversion learning and CREB phosphorylation in ratsBrain Research, 2010
- Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer MortalityMarine Biotechnology, 2009
- Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR dataNucleic Acids Research, 2009
- MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequencesBMC Genomics, 2009
- annot8r: GO, EC and KEGG annotation of EST datasetsBMC Bioinformatics, 2008
- Phosphorylation inhibits turnover of the tau protein by the proteasome: influence of RCAN1 and oxidative stressBiochemical Journal, 2006
- Development of mussel mRNA profiling: Can gene expression trends reveal coastal water pollution?Mutation Research, 2006
- Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray ExperimentsStatistical Applications in Genetics and Molecular Biology, 2004
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Analysis of Variance for Gene Expression Microarray DataJournal of Computational Biology, 2000