Complementary DNA sequence of 3-methylcholanthrene-inducible P-450 from the rat lung.

Abstract

KIKUCHI, H., SAGAMI, I., FUJII, H., OHMACHI, T. and WATANABE, M. Complementary DNA Sequence of 3-Methylcholanthrene-Inducible P-450 from the Rat Lung. Tohoku J. Exp. Med., 1990, 160 (4), 323-332-Cytochrome P-450MC was induced in pulmonary microsomes of 3-methylcholanthrene-treated rats and also at low level in that of isosafrole-treated rats. Cytochrome P-450d was not detected in the lungs of 3-methylcholanthrene- or isosafrole-treated rats by the method of Western blot analysis with a polyclonal antibody raised against cytochrome P-450c which is equally effective against P-450d, nor by the method of Northern hybridization probed with pcP450mc3 (P-450d probe). Complementary DNA of P-450MC was isolated from rat pulmonary cDNA library and the nucleotide sequence of pulmonary cDNA was compared with that of hepatic P-450c cDNA reported by Yabusaki et al. There was no gross change in nucleotide sequences of cDNA between pulmonary P-450MC and hepatic P-450c.