Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil
- 1 December 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 264 (2), 617-620
- https://doi.org/10.1042/bj2640617
Abstract
Neutrophils stimulated with formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) in the presence of butanol and ethanol formed phosphatidyl alcohols through a phospholipase D mechanism. The alcohols inhibited phosphatidic acid and diradylglycerol (DRG) formation, but did not block inositol 1, 4, 5-trisphosphate release. fMet-Leu-Phe-stimulated superoxide production was inhibited by alcohol concentrations which blocked DRG formation, whereas opsonized-zymosan-stimulated superoxide production was only partially decreased. These results suggest that phospholipase D activation is functionally linked to superoxide production in the human neutrophil.This publication has 33 references indexed in Scilit:
- Development of a novel, Ins(1,4,5)P3-specific binding assay: Its use to determine the intracellular concentration of Ins(1,4,5)P3 in unstimulated and vasopressin-stimulated rat hepatocytesCellular Signalling, 1989
- Interleukin-1 generates transmembrane signals from phospholipids through novel pathways in cultured rat mesangial cells.JCI Insight, 1989
- Phosphatidylethanol formation in human platelets: Evidence for thrombin-induced activation of phospholipase DBiochemical and Biophysical Research Communications, 1988
- 12-O-tetradecanoylphorbol-13-acetate activates phosphatidylethanol and phosphatidylglycerol synthesis by phospholipase D in cell lysatesBiochemical and Biophysical Research Communications, 1988
- Activation of phospholipase D by chemotactic peptide in HL-60 granulocytesBiochemical and Biophysical Research Communications, 1988
- Ca2 +‐mobilizing hormones elicit phosphatidylethanol accumulation via phospholipase D activationFEBS Letters, 1987
- PHOSPHATIDYLINOSITOL TURNOVER IN RECEPTOR MECHANISM AND SIGNAL TRANSDUCTIONAnnual Review of Pharmacology and Toxicology, 1985
- Intracellular translocation of phosphatidate phosphohydrolase and its possible role in the control of glycerolipid synthesisProgress in Lipid Research, 1984
- Superoxide generation by digitonin-stimulated guinea pig granulocytes. A basis for a continuous assay for monitoring superoxide production and for the study of the activation of the generating system.JCI Insight, 1978
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959