Malaria Sporozoites Release Circumsporozoite Protein from Their Apical End and Translocate It along Their Surface
- 1 July 1991
- journal article
- Published by Wiley in The Journal of Protozoology
- Vol. 38 (4), 411-421
- https://doi.org/10.1111/j.1550-7408.1991.tb01379.x
Abstract
Plasmodium sporozoites, the causative agents of malaria, release circumsporozoite (CS) protein into medium when under conditions simulating those that the parasites encounter in the bloodstream of the vertebrate host. CS protein of the rodent parasite, Plasmodium berghei, is released as the lower molecular weight form, Pb44. This release is substratum- and antibody-independent. Previous studies show that CS protein is released at the trailing, posterior end of motile sporozoites. Video and electron microscopic studies now demonstrate that CS protein is released at the apical end of cytochalasin b-immobilized sporozoites. We propose that CS protein released from the apical end, the leading end of gliding sporozoites, adheres to the sporozoite surface and is translocated posteriorly by a cytochalasin-sensitive and apparently actin-mediated surface motor, which drives gliding motility. This model explains the mechanism of both the circumsporozoite precipitation (CSP) reaction and formation of the CS protein trail by gliding sporozoites.Keywords
This publication has 46 references indexed in Scilit:
- Malaria Sporozoites Leave Behind Trails of Circumsporozoite Protein During Gliding Motility1The Journal of Protozoology, 1988
- Rhoptry Secretion of Membranous Whorls byPlasmodium bergheiSporozoites1The Journal of Protozoology, 1985
- Molecular Approaches to Malaria VaccinesScientific American, 1985
- The mechanism of diatom locomotion. I. An ultrastructural study of the motility apparatusProceedings of the Royal Society of London. B. Biological Sciences, 1983
- Substrate Contact, Mucus, and Eugregregarine Gliding1The Journal of Protozoology, 1983
- Sporozoites of Mammalian Malaria: Attachment To, Interiorization and Fate Within MacrophagesThe Journal of Protozoology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Studies on the Motility of Plasmodium Sporozoites*The Journal of Protozoology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Diatom locomotion: An explanationProtoplasma, 1966