A Confirmation of the Presence of Pancreozymin in the Duodenal Mucosa

Abstract

The volume and enzyme responses of cannulated pancreatic juice from fasted mongrel doga differed after inj. of secretin concentrates. Purified secretin evoked an enzyme-poor secretion. Pure secretin plus the "AP" fraction markedly increased the content of all enzymes. The AP fraction contained a principle (pancreozymin) which stimulated pancreatic en- zyme flow. This was nullified after incubation with dog serum, indicating that blood contained an enzyme substance which inactivated pancreozymin. Secretin and pan-creozymin were separated by ppt. of the latter with aniline.