Deuterium Induced Extinction of ADH-release in Response to Intracerebroventricular Infusions of Hypertonic NaCl and Angiotensin

Abstract

Infusions (20μl/min) of hypertonic (0.3 M) NaCl and angiotensin 11 (1 ng/kg min^‐1)in isotonic (0.15 M) NaCl were made for 1 h in the hydrated goat during fully developed water diuresis. Either H₂O or deuterium (D₂O) was used as solvent. A pronounced antidiuretic response, outlasting the infusion period by 30 min or more, was seen when the substances were dissolved in H₂O. Only a weak inhibition of the water diuresis, which was extinguished during the infusion period, was obtained when D₂O was used as the solvent. The infusion of 0.3 M NaCl/H₂O invariably induced drinking in one of the goats, which, however, showed no drinking response to the infusions of 0.3 M NaCl/D₂O. The possibility is discussed that D₂O (perhaps by‐its inhibitory effect on (Na+‐K+)‐ATPase activity) reduced the sensitivity of juxtaventricular receptors regulating ADH‐release and water intake.