CLOCK, an essential pacemaker component, controls expression of the circadian transcription factor DBP
- 15 March 2000
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 14 (6), 679-689
- https://doi.org/10.1101/gad.14.6.679
Abstract
DBP, the founding member of the PAR leucine zipper transcription factor family, is expressed according to a robust daily rhythm in the suprachiasmatic nucleus and several peripheral tissues. Previous studies with mice deleted for the Dbp gene have established that DBP participates in the regulation of several clock outputs, including locomotor activity, sleep distribution, and liver gene expression. Here we present evidence that circadian Dbptranscription requires the basic helix–loop–helix–PAS protein CLOCK, an essential component of the negative-feedback circuitry generating circadian oscillations in mammals and fruit flies. Genetic and biochemical experiments suggest that CLOCK regulates Dbpexpression by binding to E-box motifs within putative enhancer regions located in the first and second introns. Similar E-box motifs have been found previously in the promoter sequence of the murine clock genemPeriod1. Hence, the same molecular mechanisms generating circadian oscillations in the expression of clock genes may directly control the rhythmic transcription of clock output regulators such asDbp.Keywords
This publication has 57 references indexed in Scilit:
- Role of the CLOCK Protein in the Mammalian Circadian MechanismScience, 1998
- The basic-helix–loop–helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factorsProceedings of the National Academy of Sciences, 1998
- Bacterial Cell Wall Polymer-Induced Granulomatous InflammationMethods, 1996
- Immunochemical Characterization and Transacting Properties of Upstream Stimulatory Factor IsoformsPublished by Elsevier BV ,1996
- Transcription Factors Stat3 and Stat5b Are Present in Rat Liver Nuclei Late in an Acute Phase Response and Bind Interleukin-6 Response ElementsPublished by Elsevier BV ,1995
- Hlf, a novel hepatic bZIP protein, shows altered DNA-binding properties following fusion to E2A in t(17;19) acute lymphoblastic leukemia.Genes & Development, 1992
- Fusion of the Leucine Zipper Gene HLF to the E2A Gene in Human Acute B-Lineage LeukemiaScience, 1992
- Expression of the liver-enriched transcriptional activator protein DBP follows a stringent circadian rhythmCell, 1990
- Transplanted Suprachiasmatic Nucleus Determines Circadian PeriodScience, 1990
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970