Large-scale isolation of equine liver alcohol dehydrogenase on a blue agarose gel

Abstract

Equine liver alcohol dehydrogenase (EC 1.1.1.1) has been purified by a new scheme using a blue agarose gel (Blue Sepharose) as an affinity sorbent. Starting amounts of 0.6 to 10 kg liver have been processed to enzyme possessing 1.5 U/mg average specific activity, in about three to four days. Some parameters concerning adsorption of enzyme to the blue gel as well as recovery therefrom have been explored.