EARLY ENZYME SYNTHESIS AND ITS CONTROL IN E. COLI INFECTED WITH SOME AMBER MUTANTS OF BACTERIOPHAGE T4

Abstract

Amber mutants of bacteriophage-T4 inducing phage production in E. coli K-12 strains but not in E. coli B have been examined for ability to induce the formation of enzyme activities related to DNA synthesis in E. coll. Two of seven mutants were shown to be incapable of inducing DNA synthesis and abnormal kinetics were obtained with three others. The inability to induce DNA synthesis has been attributed in one instance to lack of appearance of deoxycytidylate hydroxymethylase and phage-induced DNA polymerase.