Glycolysis and glycogen metabolism after inhibition of hexose monophosphate pathway in C6-GLIAL cells

Abstract

6-Aminonicotinamide (0.01 mg/ml) leads to a strong accumulation of 6-PG in C6 glial cells after 24 h. The accumulated 6-PG is dephosphorylated to gluconate which easily permeates the cell membrane. Extracellular gluconate is formed at a rate of 12% of the total glucose consumption. 6-PG as competitive inhibitor of the PGI caused a reduction of the glycolytic flux of about 40%. The reduced glycolytic flux lowers the ATP concentration under anaerobic conditions to 75% of the controls. The glycogen content after 6-AN is increased by 50%, probably by the activation of the glycogen synthetase due to the higher Glc 6-P concentration. The fibroblast-like morphology of the C6 cell line has typically changed under 6-aminonicotinamide.