Gene Dosage for 5 S Ribosomal Ribonucleic Acid in Escherichia coli and Bacillus megaterium
Open Access
- 1 January 1971
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 105 (1), 142-149
- https://doi.org/10.1128/jb.105.1.142-149.1971
Abstract
The number of gene copies for 5 S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23 S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium . In both cases, the number of 5 S rRNA genes equals the number of 16 or 23 S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5 S rRNA are described.Keywords
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