Biological and biochemical observations on isolates of EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas
- 15 September 1979
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 24 (3), 294-302
- https://doi.org/10.1002/ijc.2910240305
Abstract
Foetal, adult seronegative, and cotton-top marmoset lymphocytes have been transformed into cell lines by EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas. The nature of the cell lines was checked by karyotyping and by light and electron microscopy, and the presence of the EB virus genome was demonstrated by immunofluorescence tests. Immunofluorescence also showed the incidence of EB virus-producing cells in each line and this was checked by electron microscopy and the use of a lymphocyte transformation assay. Two foetal-derived lines did not produce virus spontaneously, could not be activated with various inducers, and were found by DNA reassociation kinetics to carry only small numbers of genome copies per cell. An adult-derived line produced virus, could be activated to produce more, and provided enough infectivity to transform marmoset cells. The resulting marmoset line made profuse transforming virus; it thus provides the first abundant source of NPC-derived infectious EB virus for comparative studies. The results are discussed in relation to the interactions with comparable target cells of EB virus from normal individuals and from patients with other diseases.This publication has 50 references indexed in Scilit:
- Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude-mouse-passaged nasopharyngeal carcinomaInternational Journal of Cancer, 1979
- The Epstein-Barr VirusPublished by Springer Science and Business Media LLC ,1979
- Epidemiological evidence for causal relationship between Epstein-Barr virus and Burkitt's lymphoma from Ugandan prospective studyNature, 1978
- Epstein-Barr virus as the cause of a human cancerNature, 1978
- RECENT PROGRESS IN EPSTEIN-BARR VIRUS RESEARCHAnnual Review of Microbiology, 1977
- Heterogeneity of epstein‐barr virus originating from P3HR‐1 cells. I. Studies on ebna inductionInternational Journal of Cancer, 1977
- C-type virus particles in human tumours transplanted into nude miceBritish Journal of Cancer, 1976
- Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV)-genome-negative human lymphoma cells with different EBV strainsInternational Journal of Cancer, 1976
- Pilot experiments with EB virus in owl monkeys (aotus trivirgatus). II. EB virus in a cell line from an animal with reticuloproliferative diseaseInternational Journal of Cancer, 1973
- The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel FiltrationScandinavian Journal of Clinical and Laboratory Investigation, 1968