Diversity of human colonic butyrate‐producing bacteria revealed by analysis of the butyryl‐CoA:acetate CoA‐transferase gene
- 26 January 2010
- journal article
- Published by Wiley in Environmental Microbiology
- Vol. 12 (2), 304-314
- https://doi.org/10.1111/j.1462-2920.2009.02066.x
Abstract
Butyrate-producing bacteria play an important role in the human colon, supplying energy to the gut epithelium and regulating host cell responses. In order to explore the diversity and culturability of this functional group, we designed degenerate primers to amplify butyryl-CoA:acetate CoA-transferase sequences from faecal samples provided by 10 healthy volunteers. Eighty-eight per cent of amplified sequences showed >98% DNA sequence identity to CoA-transferases from cultured butyrate-producing bacteria, and these fell into 12 operational taxonomic units (OTUs). The four most prevalent OTUs corresponded to Eubacterium rectale, Roseburia faecis, Eubacterium hallii and an unnamed cultured species SS2/1. The remaining 12% of sequences, however, belonged to 20 OTUs that are assumed to come from uncultured butyrate-producing strains. Samples taken after ingestion of inulin showed significant (P=0.019) increases in Faecalibacterium prausnitzii. Because several of the dominant butyrate producers differ in their DNA % G+C content, analysis of thermal melt curves obtained for PCR amplicons of the butyryl-CoA:acetate CoA-transferase gene provides a convenient and rapid qualitative assessment of the major butyrate producing groups present in a given sample. This type of analysis therefore provides an excellent source of information on functionally important groups within the colonic microbial community.Keywords
This publication has 40 references indexed in Scilit:
- Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patientsProceedings of the National Academy of Sciences of the United States of America, 2008
- Comparative Metagenomics Revealed Commonly Enriched Gene Sets in Human Gut MicrobiomesDNA Research, 2007
- Development of a Semiquantitative Degenerate Real-Time PCR-Based Assay for Estimation of Numbers of Butyryl-Coenzyme A (CoA) CoA Transferase Genes in Complex Bacterial SamplesApplied and Environmental Microbiology, 2007
- Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in FecesApplied and Environmental Microbiology, 2007
- The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public dataNucleic Acids Research, 2006
- Molecular Diversity, Cultivation, and Improved Detection by Fluorescent In Situ Hybridization of a Dominant Group of Human Gut Bacteria Related to Roseburia spp. or Eubacterium rectaleApplied and Environmental Microbiology, 2006
- Metagenomic Analysis of the Human Distal Gut MicrobiomeScience, 2006
- Host-Bacterial Mutualism in the Human IntestineScience, 2005
- Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation ProductApplied and Environmental Microbiology, 2004
- Multiple sequence alignment with the Clustal series of programsNucleic Acids Research, 2003