Next-generation sequencing: applications beyond genomes
Open Access
- 19 September 2008
- journal article
- Published by Portland Press Ltd. in Biochemical Society Transactions
- Vol. 36 (5), 1091-1096
- https://doi.org/10.1042/bst0361091
Abstract
The development of DNA sequencing more than 30 years ago has profoundly impacted biological research. In the last couple of years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of complex samples at unprecedented scale and speed. These next-generation technologies make it feasible to sequence not only static genomes, but also entire transcriptomes expressed under different conditions. These and other powerful applications of next-generation sequencing are rapidly revolutionizing the way genomic studies are carried out. Below, we provide a snapshot of these exciting new approaches to understanding the properties and functions of genomes. Given that sequencing-based assays may increasingly supersede microarray-based assays, we also compare and contrast data obtained from these distinct approaches.This publication has 56 references indexed in Scilit:
- Mapping and quantifying mammalian transcriptomes by RNA-SeqNature Methods, 2008
- Highly Integrated Single-Base Resolution Maps of the Epigenome in ArabidopsisCell, 2008
- The complete genome of an individual by massively parallel DNA sequencingNature, 2008
- High-Resolution Mapping and Characterization of Open Chromatin across the GenomeCell, 2008
- Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termiteNature, 2007
- Genome-wide maps of chromatin state in pluripotent and lineage-committed cellsNature, 2007
- Genome-wide transcription and the implications for genomic organizationNature Reviews Genetics, 2007
- High-Resolution Profiling of Histone Methylations in the Human GenomeCell, 2007
- High-throughput oncogene mutation profiling in human cancerNature Genetics, 2007
- Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBFNature, 2001