New Species of Madurella, Causative Agents of Black-Grain Mycetoma
- 1 March 2012
- journal article
- case report
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 50 (3), 988-994
- https://doi.org/10.1128/jcm.05477-11
Abstract
A new species of nonsporulating fungus, isolated in a case of black-grain mycetoma in Sudan, is described as Madurella fahalii . The species is characterized by phenotypic and molecular criteria. Multigene phylogenies based on the ribosomal DNA (rDNA) internal transcribed spacer (ITS), the partial β-tubulin gene ( BT2 ), and the RNA polymerase II subunit 2 gene ( RPB2 ) indicate that M. fahalii is closely related to Madurella mycetomatis and M. pseudomycetomatis ; the latter name is validated according to the rules of botanical nomenclature. Madurella ikedae was found to be synonymous with M. mycetomatis . An isolate from Indonesia was found to be different from all known species based on multilocus analysis and is described as Madurella tropicana. Madurella is nested within the order Sordariales, with Chaetomium as its nearest neighbor. Madurella fahalii has a relatively low optimum growth temperature (30°C) and is less susceptible to the azoles than other Madurella species, with voriconazole and posaconazole MICs of 1 μg/ml, a ketoconazole MIC of 2 μg/ml, and an itraconazole MIC of >16 μg/ml. Since eumycetoma is still treated only with azoles, correct species identification is important for the optimal choice of antifungal therapy.Keywords
This publication has 16 references indexed in Scilit:
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- Madurella pseudomycetomatis: an Invalidly Published NameJournal of Clinical Microbiology, 2011
- Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain MycetomaJournal of Clinical Microbiology, 2010
- Management of mycetoma: major challenge in tropical mycoses with limited international recognitionCurrent Opinion in Infectious Diseases, 2007
- Molecular Identification of Black-Grain Mycetoma AgentsJournal of Clinical Microbiology, 2006
- Multi-gene phylogenies indicate ascomal wall morphology is a better predictor of phylogenetic relationships than ascospore morphology in the Sordariales (Ascomycota, Fungi)Molecular Phylogenetics and Evolution, 2005
- In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2 H - Tetrazolium Hydroxide (XTT) AssayAntimicrobial Agents and Chemotherapy, 2004
- Phylogeny and typification of Madurella mycetomatis, with a comparison of other agents of eumycetomaMycoses, 2004
- Molecular Detection and Identification of Agents of Eumycetoma: Detailed Report of Two CasesJournal of Clinical Microbiology, 2003
- Recent Taxonomic Developments and Changes in Medical MycologyAnnual Review of Microbiology, 1980