A Single Monoclonal Antibody against the Peanut Allergen Ara h 2 Protects against Systemic and Local Peanut Allergy
Open Access
- 1 May 2020
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 181 (5), 334-341
- https://doi.org/10.1159/000505917
Abstract
Background: Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible. Methods: The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test. Results: Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity Fc gamma RIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on Fc gamma RIIb. Conclusion: A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.Keywords
This publication has 21 references indexed in Scilit:
- IL-10 and Regulatory T Cells Cooperate in Allergen-Specific Immunotherapy To Ameliorate Allergic AsthmaPublished by The American Association of Immunologists ,2015
- Measuring Local Anaphylaxis in MiceJournal of Visualized Experiments, 2014
- Detection of Parasite Antigens inLeishmania infantum–Infected Spleen Tissue by Monoclonal Antibody-, Piezoelectric-Based ImmunosensorsJournal of Parasitology, 2014
- The epidemiology of food allergy in Europe: a systematic review and meta‐analysisAllergy, 2013
- Multiple-allergen and single-allergen immunotherapy strategies in polysensitized patients: Looking at the published evidenceJournal of Allergy and Clinical Immunology, 2012
- Mechanisms of allergen-specific desensitizationJournal of Allergy and Clinical Immunology, 2010
- The psychosocial impact of food allergy and food hypersensitivity in children, adolescents and their families: a reviewAllergy, 2010
- Displaying Fel d1 on virus-like particles prevents reactogenicity despite greatly enhanced immunogenicity: a novel therapy for cat allergyThe Journal of Experimental Medicine, 2009
- The natural progression of peanut allergy: Resolution and the possibility of recurrenceJournal of Allergy and Clinical Immunology, 2003
- Quantification of major peanut allergens Ara h 1 and Ara h 2 in the peanut varieties Runner, Spanish, Virginia, and Valencia, bred in different parts of the worldAllergy, 2001