The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and α
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (12), 4185-4193
- https://doi.org/10.1093/nar/11.12.4185
Abstract
The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.Keywords
This publication has 21 references indexed in Scilit:
- Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.Proceedings of the National Academy of Sciences, 1978
- Comparative utilization of bromodeoxyuridine and iododeoxyuridine triphosphates for mammalian DNA replication in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I.Journal of Biological Chemistry, 1978
- Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-sNucleic Acids Research, 1977
- DNA synthesis with methylated poly(dA-dT) templates: possible role of O4-methylthymine as a pro-mutagenic baseNucleic Acids Research, 1977
- Cellular reactions of O6-methylguanine, a product of some alkylating carcinogensBiochemical Journal, 1973
- Reaction products from N-methyl-N-nitrosourea and deoxyribonucleic acid containing thymidine residues. Synthesis and identification of a new methylation product, O4-methyl-thymidineBiochemical Journal, 1973
- The properties of
methylguanine in templates for RNA polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973 - Synthesis and chemical properties of monomers and polymers containing 7-methylguanine and an investigation of their substrate or template properties for bacterial deoxyribonucleic acid or ribonucleic acid polymerasesBiochemistry, 1970
- Possible Relevance of O–6 Alkylation of Deoxyguanosine to the Mutagenicity and Carcinogenicity of Nitrosamines and NitrosamidesNature, 1969