Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli
Open Access
- 15 March 2009
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (6), 1628-1634
- https://doi.org/10.1128/aem.02376-08
Abstract
In an effort to improve industrial production of 1,3-propanediol (1,3-PD), we engineered a novel polycistronic operon under the control of the temperature-sensitive lambda phage P L P R promoter regulated by the c I ts 857 repressor and expressed it in Escherichia coli K-12 ER2925. The genes for the production of 1,3-PD in Clostridium butyricum , dhaB1 and dhaB2 , which encode the vitamin B 12 -independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, were tandemly arrayed with the E. coli yqhD gene, which encodes the 1,3-propanediol oxidoreductase isoenzyme YqhD, an NADP-dependent dehydrogenase that can directly convert glycerol to 1,3-PD. The microbial conversion of 1,3-PD from glycerol by this recombinant E. coli strain was studied in a two-stage fermentation process. During the first stage, a novel high-cell-density fermentation step, there was significant cell growth and the majority of the metabolites produced were organic acids, mainly acetate. During the second stage, glycerol from the fresh medium was rapidly converted to 1,3-PD following a temperature shift from 30°C to 42°C. The by-products were mainly pyruvate and acetate. During this two-stage process, the overall 1,3-PD yield and productivity reached 104.4 g/liter and 2.61 g/liter/h, respectively, and the conversion rate of glycerol to 1,3-PD reached 90.2% (g/g). To our knowledge, this is the highest reported yield and productivity efficiency of 1,3-PD with glycerol as the sole source of carbon. Furthermore, the overall fermentation time was only 40 h, shorter than that of any other reports.Keywords
This publication has 32 references indexed in Scilit:
- Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01Journal of Industrial Microbiology & Biotechnology, 2008
- Microbial production of 1,3-propanediol from glycerol by Klebsiella pneumoniae under micro-aerobic conditions up to a pilot scaleBiotechnology Letters, 2007
- Cloning and Expression of Human Stem Cell Factor Fused with Thrombopoietin Mimetic Peptide in Escherichia coliBiotechnology Letters, 2006
- Improved glutathione production by gene expression in Escherichia coliLetters in Applied Microbiology, 2006
- Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genesFEMS Microbiology Reviews, 1998
- Protein staining by Coomassie Brilliant Blue G-250: Unique color rendering in iron-toned printsElectrophoresis, 1986
- Mutants of Escherichia coli K12 with Defects in Anaerobic Pyruvate MetabolismMicrobiology, 1981
- Role of peripheral side chains of vitamin B12 coenzymes in the reaction catalyzed by dioldehydraseBiochemistry, 1979
- Maximal exponential growth rate and yield of E. coli obtainable in a bench‐scale fermentorBiotechnology & Bioengineering, 1974
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961