Selectins and neutrophil traffic: margination and Streptococcus pneumoniae-induced emigration in murine lungs.
Open Access
- 1 August 1996
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 184 (2), 639-645
- https://doi.org/10.1084/jem.184.2.639
Abstract
The roles of selectins in the pulmonary margination and emigration of neutrophils were investigated by using mice genetically deficient in both E- and P-selectins (E/P mutants) and/or by intravenous injections of fucoidin (inhibiting both L- and P-selectins). E/P mutants were neutrophilic (14.7 +/- 4.9 x 10(6) vs. 0.8 +/- 0.1 x 10(6) neutrophils/ml). This neutrophilia was associated with increased margination of neutrophils within pulmonary capillaries (39.7 +/- 9.4 vs. 4.6 +/- 1.1 neutrophil profiles per 100 red blood cell profiles) but no change in margination within noncapillary pulmonary microvessels. After intratracheal instillation of Streptococcus pneumoniae, lungs of E/P mutants displayed increased neutrophil emigration (564 +/- 92 vs. 116 +/- 19 neutrophils per 100 alveolar profiles), edema (5.3 +/- 1.5 vs. 1.5 +/- 0.4 microliter/g body weight), and histologic evidence of lung injury compared with those in wild-type (WT). Fucoidin treatment did not affect neutrophil emigration during streptococcal pneumonia in WT or E/P mice. During pneumonia, the number of white blood cells (WBC) tethered to or spread upon the noncapillary vessel endothelium increased in both WT and E/P lungs. These are the first data demonstrating that neutrophil margination in uninfected pulmonary capillaries does not require E- and P-selectins; that streptococcal pneumonia induces an E- and P-selectin-independent increase in WBC interactions with noncapillary endothelium; and that migration of neutrophils to alveoli can occur despite deficiency or inhibition of all of the known selectins.Keywords
This publication has 27 references indexed in Scilit:
- Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice.The Journal of Experimental Medicine, 1996
- Quantitation of neutrophil migration in acute bacterial pneumonia in rabbitsJournal of Applied Physiology, 1994
- Protective effects of sialylated oligosaccharides in immune complex-induced acute lung injury.The Journal of Experimental Medicine, 1993
- Comparison of neutrophil and capillary diameters and their relation to neutrophil sequestration in the lungJournal of Applied Physiology, 1993
- Fucoidin, but not yeast polyphosphomannan PPME, inhibits leukocyte rolling in venules of the rat mesentery.1993
- Neutrophil Sequestration and Migration in Localized Pulmonary Inflammation: Capillary Localization and Migration across the Interalveolar SeptumAmerican Review of Respiratory Disease, 1993
- Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats.JCI Insight, 1991
- Adhesion protein GMP140 inhibits superoxide anion release by human neutrophils.Proceedings of the National Academy of Sciences, 1991
- CD18-dependent and -independent mechanisms of neutrophil emigration in the pulmonary and systemic microcirculation of rabbits.The Journal of Immunology, 1990
- Mechanics of Stimulated Neutrophils: Cell Stiffening Induces Retention in CapillariesScience, 1989