Nanogyroids Incorporating Multivalent Lipids: Enhanced Membrane Charge Density and Pore Forming Ability for Gene Silencing
- 25 May 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 27 (12), 7691-7697
- https://doi.org/10.1021/la200679x
Abstract
The self-assembly of a custom-synthesized pentavalent cationic lipid (MVL5) and glycerol monooleate (GMO) with small interfering RNA (siRNA) results in the formation of a double-gyroid bicontinuous inverted cubic phase with colocalized lipid/siRNA domains as shown by synchrotron X-ray scattering and fluorescence microscopy. The high charge density (due to MVL5) and positive Gaussian modulus of the GMO-containing membranes confer optimal electrostatic and elastic properties for endosomal escape, enabling efficient siRNA delivery and effective, specific gene silencing.Keywords
This publication has 41 references indexed in Scilit:
- Highly Efficient Gene Silencing Activity of siRNA Embedded in a Nanostructured Gyroid Cubic Lipid MatrixJournal of the American Chemical Society, 2010
- Gene Regulation with Polyvalent siRNA−Nanoparticle ConjugatesJournal of the American Chemical Society, 2009
- Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1JCI Insight, 2008
- Aptamer mediated siRNA deliveryNucleic Acids Research, 2006
- Unlocking the potential of the human genome with RNA interferenceNature, 2004
- Therapeutic siRNAsTrends in Pharmacological Sciences, 2004
- Gene Therapy--New Challenges AheadScience, 2003
- Progress and problems with the use of viral vectors for gene therapyNature Reviews Genetics, 2003
- Observation of a Membrane Fusion Intermediate StructureScience, 2002
- Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in transPlant Cell, 1990